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Addgene

piRFP720-N1 Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb Extend DNA ladder and a digestion of plasmid 45461 with AgeI-HF and NotI-HF.

We expect and see the following banding pattern:

AgeI-HF single digest: Expect 1 band: ~5kb
AgeI-HF / NotI-HF double digest: Expect 2 bands: ~4 and 1 kb