pR6Kfrt-gent-frt
(Plasmid #45469)

• Depositing Lab: Michele Swanson
• Publication: Bryan et al Appl Environ Microbiol. 2011 Apr;77(7):2545-8. doi: 10.1128/AEM.02904-10. Epub 2011 Feb 4.

Backbone

• Vector backbone: pR6K
• Backbone manufacturer: Datensko and Wanner
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5a lambda pir
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: frt-gent-frt
• Alt name: MB838

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: R6K-3'

Resource Information

• Supplemental Documents:
  • genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

frt-gent-frt cassette was cut out of pGEM-frt-gent-frt with EcoRI and ligated into EcoRI digested pR6K. Please note that the R6K ori may be in opposite orientation relative to cassette and annotation since it was not directionally cloned.