JAF99.4
(Plasmid
#45560)
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Depositing Lab
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Depositing OrganizationUniversity of Massachusetts, Worcester
Located in United States of America -
Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45560 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 45560-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 45560-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C2
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePkhd1
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SpeciesM. musculus (mouse)
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MutationContains only the cytoplasmic tail (last 193 amino acids)
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GenBank IDNM_153179.2
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Entrez GenePkhd1 (a.k.a. FPC, Tigm1)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 45560-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 45560-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JAF99.4 was a gift from Gregory Pazour (Addgene plasmid # 45560 ; http://n2t.net/addgene:45560 ; RRID:Addgene_45560) -
For your References section:
The cytoplasmic tail of fibrocystin contains a ciliary targeting sequence. Follit JA, Li L, Vucica Y, Pazour GJ. J Cell Biol. 2010 Jan 11;188(1):21-8. doi: 10.1083/jcb.200910096. Epub 2010 Jan 4. 10.1083/jcb.200910096 PubMed 20048263