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(Plasmid #40232)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40232 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    SNAP25 (a.k.a. CMS18, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, bA416N4.2, dJ1068F16.2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Venus (C terminal on insert)
    • CFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI, BspEI, AgeI, XhoI (unknown if destroyed)
  • 3′ cloning site BcII (unknown if destroyed)
  • 5′ sequencing primer CMV sequence
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene found E713 in the SCORE sensor was Alanine when compared to the depositor's sequence. This result matches the expected Snap25 sequence (GenBank IDNM_130811).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SCORE was a gift from Wolfhard Almers (Addgene plasmid # 40232 ; ; RRID:Addgene_40232)
  • For your References section:

    Tracking SNARE complex formation in live endocrine cells. An SJ, Almers W. Science. 2004 Nov 5;306(5698):1042-6. 10.1126/science.1102559 PubMed 15528447