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SCORE
(Plasmid #40232)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40232 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 40232-D50 50 μg of DNA in Tris buffer $495
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DNA 40232-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    peYFP-N1
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Snap25b
  • Alt name
    score
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2164
  • GenBank ID
    NM_130811
  • Entrez Gene
    SNAP25 (a.k.a. CMS18, DEE117, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, bA416N4.2, dJ1068F16.2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Venus (C terminal on insert)
    • CFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI, BspEI, AgeI, XhoI (unknown if destroyed)
  • 3′ cloning site BcII (unknown if destroyed)
  • 5′ sequencing primer CMV sequence
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene found E713 in the SCORE sensor was Alanine when compared to the depositor's sequence. This result matches the expected Snap25 sequence (GenBank IDNM_130811).

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SCORE was a gift from Wolfhard Almers (Addgene plasmid # 40232 ; http://n2t.net/addgene:40232 ; RRID:Addgene_40232)

  • For your References section:

    Tracking SNARE complex formation in live endocrine cells. An SJ, Almers W. Science. 2004 Nov 5;306(5698):1042-6. 10.1126/science.1102559 PubMed 15528447
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