Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pD40-His/V5-c-Myc
(Plasmid #45597)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45597 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA DEST40
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7143
  • Total vector size (bp) 6841
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-Myc
  • Alt name
    Myc2
  • Alt name
    myelocytomatosis oncogene
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1400
  • Entrez Gene
    Myc (a.k.a. AU016757, Myc2, N, Niard, Nird, bHLHe3, bHLHe39)
  • Promoter CMV
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6xHis (C terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Murine c-myc cDNA amplified from plasmid CMV-Myc from Stephen R. Hann (Vanderbuilt University) (PMID: 7958908) (Murine myc cDNA originally obtained from Michael Cole, Princeton, NJ)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Alternate plasmid names: pD40-His-c-Myc; pcDNA-DEST40 Myc wt

This His6-tagged c-Myc plasmid was created by PCR amplification of the coding sequence for wild-type murine c-Myc2 from the CMVMyc plasmid (Hann et al., Genes Dev. 1994, PMID: 7958908) with a CACC 5′ extension added to the 5′ primer for cloning into the TOPO entry clone from Invitrogen (Carlsbad, CA). This sequence was then moved into the pDEST-40 mammalian expression vector using Gateway technology (Invitrogen), creating pD40-His-c-Myc.

For plasmid usage information, see the associated article (Yeh et al. Nat Cell Biol. 2004) as well as Arnold et al. EMBO 2009 (PMID: 19131971).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pD40-His/V5-c-Myc was a gift from Rosalie Sears (Addgene plasmid # 45597 ; http://n2t.net/addgene:45597 ; RRID:Addgene_45597)
  • For your References section:

    A signalling pathway controlling c-Myc degradation that impacts oncogenic transformation of human cells. Yeh E, Cunningham M, Arnold H, Chasse D, Monteith T, Ivaldi G, Hahn WC, Stukenberg PT, Shenolikar S, Uchida T, Counter CM, Nevins JR, Means AR, Sears R. Nat Cell Biol. 2004 Apr;6(4):308-18. Epub 2004 Mar 14. 10.1038/ncb1110 PubMed 15048125