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pET28b-Ava3856
(Plasmid #45742)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45742 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5293
  • Total vector size (bp) 6670
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ava3856
  • Alt name
    ATP-grasp enzyme
  • Alt name
    Ava_3856
  • Species
    Anabaena variabilis
  • Insert Size (bp)
    1377
  • GenBank ID
    YP_324356
  • Entrez Gene
    Ava_3856 (a.k.a. Ava_3856)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-Term
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-Ava3856 was a gift from Emily Balskus & Christopher T Walsh (Addgene plasmid # 45742 ; http://n2t.net/addgene:45742 ; RRID:Addgene_45742)
  • For your References section:

    The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Balskus EP, Walsh CT. Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637. Epub 2010 Sep 2. 10.1126/science.1193637 PubMed 20813918