TN-XXL pcDNA3
(Plasmid #45797)

• Purpose: Fluorescent reporter for calcium signaling
• Depositing Lab: Oliver Griesbeck
• Publication: Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi: 10.1038/nmeth.1243.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TN-XXL
• Species: G. gallus (chicken)
• Insert Size (bp): 1885
• Promoter: CMV
• Tags / Fusion Proteins:
  • ECFP (N terminal on insert)
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry