|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46004||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5047
- Total vector size (bp) 5108
Modifications to backboneInserted L3S2P21 terminator between the EcoRI and SpeI sites.
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameL3S2P21 Terminator
Insert Size (bp)61
- Promoter PBAD
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer gtccacacaatctgcccttt (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGR-L3S2P21 was a gift from Christopher Voigt (Addgene plasmid # 46004 ; http://n2t.net/addgene:46004 ; RRID:Addgene_46004)
For your References section:Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Chen YJ, Liu P, Nielsen AA, Brophy JA, Clancy K, Peterson T, Voigt CA. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515. 10.1038/nmeth.2515 PubMed 23727987