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pMT mir-1-1 reporter hsa-mir-205
(Plasmid #46744)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46744 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMT-DEST48 mir-1-1 reporter
  • Backbone manufacturer
    Bartel lab
  • Backbone size w/o insert (bp) 5179
  • Vector type
    Insect Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hsa-mir-205
  • Alt name
    hsa-mir-205 pri-mRNA
  • Species
    H. sapiens (human)
  • Entrez Gene
    MIR205 (a.k.a. MIRN205, mir-205)
  • Promoter Metallothionein (MT)
  • Tags / Fusion Proteins
    • hsa-mir-1-1 (C terminal on insert)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer MT-Forward (5'-CATCTCAGTGCAACTAAA-3')
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT mir-1-1 reporter hsa-mir-205 was a gift from David Bartel (Addgene plasmid # 46744 ; http://n2t.net/addgene:46744 ; RRID:Addgene_46744)
  • For your References section:

    Beyond Secondary Structure: Primary-Sequence Determinants License Pri-miRNA Hairpins for Processing. Auyeung VC, Ulitsky I, McGeary SE, Bartel DP. Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031. 10.1016/j.cell.2013.01.031 PubMed 23415231