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pBABE-Puro-KRas*
(Plasmid #46745)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46745 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE-Puro
  • Backbone size w/o insert (bp) 5169
  • Total vector size (bp) 5741
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KRAS*
  • Alt name
    V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    cDNA is encoded by a chimeric mixture of HRAS and KRAS codons that encode KRas protein
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-Puro-KRas* was a gift from Christopher Counter (Addgene plasmid # 46745 ; http://n2t.net/addgene:46745 ; RRID:Addgene_46745)
  • For your References section:

    Rare codons regulate KRas oncogenesis. Lampson BL, Pershing NL, Prinz JA, Lacsina JR, Marzluff WF, Nicchitta CV, MacAlpine DM, Counter CM. Curr Biol. 2013 Jan 7;23(1):70-5. doi: 10.1016/j.cub.2012.11.031. Epub 2012 Dec 13. 10.1016/j.cub.2012.11.031 PubMed 23246410