pGL3-24p3/LCN2-282C/EBP mut-LUC
(Plasmid
#46868)
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Purposemurine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporter
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Depositing Lab
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Depositing OrganizationUniversity at Buffalo (SUNY Buffalo)
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46868 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 46868-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 46868-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL3-basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4818
- Total vector size (bp) 5100
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name24p3/lipocalin 2 proximal promoter
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Alt nameLCN2 promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)282
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MutationCEBP site is mutated (see comments)
- Promoter 24p3 promoter (inactive)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Mlu I (not destroyed)
- 3′ cloning site Xho I (destroyed during cloning)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Primers used to create the C/EBP mutation were (lower case are mutations):
5-CAG CCC TTC CTG gca CTt ggC CTT GCA CAG TTC CGA C-3 and
5-AAC TGT GCA AGG cca AGt gcC AGG AAG GGC TGC AGG G-3
DNA (Catalog # 46868-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 46868-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-24p3/LCN2-282C/EBP mut-LUC was a gift from Sarah Gaffen (Addgene plasmid # 46868 ; http://n2t.net/addgene:46868 ; RRID:Addgene_46868) -
For your References section:
Distinct functional motifs within the IL-17 receptor regulate signal transduction and target gene expression. Maitra A, Shen F, Hanel W, Mossman K, Tocker J, Swart D, Gaffen SL. Proc Natl Acad Sci U S A. 2007 May 1;104(18):7506-11. Epub 2007 Apr 24. 10.1073/pnas.0611589104 PubMed 17456598