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Depositing Lab
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Depositing OrganizationINSERM, ADR Paris VII, St. Lazare (Inactive)
Located in France -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28225 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 28225-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 28225-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL3-basic
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Backbone manufacturerpromega
- Backbone size w/o insert (bp) 4817
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLCN2 promoter
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SpeciesH. sapiens (human)
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Insert Size (bp)1233
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GenBank ID
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Entrez GeneLCN2 (a.k.a. 24p3, MSFI, NGAL, p25)
- Promoter LCN2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer REVprimer3
- 3′ sequencing primer LucNRev
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byhuman promoter cloned by the provider
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 28225-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 28225-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p-LCN2 was a gift from Sebastien Jauliac (Addgene plasmid # 28225 ; http://n2t.net/addgene:28225 ; RRID:Addgene_28225) -
For your References section:
NFAT3 transcription factor inhibits breast cancer cell motility by targeting the Lipocalin 2 gene. Fougere M, Gaudineau B, Barbier J, Guaddachi F, Feugeas JP, Auboeuf D, Jauliac S. Oncogene. 2010 Apr 15. 29(15):2292-301. 10.1038/onc.2009.499 PubMed 20101218
Map uploaded by the depositor.
