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pDL278
(Plasmid #46882)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46882 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDL276
  • Backbone manufacturer
    Addgene plasmid 46881
  • Backbone size (bp) 6733
  • Modifications to backbone
    Kanamycin resistance-encoding aphA3 gene and pVA380-1 ORF-1 of pDL276 replaced with spectinomycin resistance-encoding aad9 gene and pVA380-1 ORF-1 of pDL602 (see associated article for detailed construction information).
  • Vector type
    Bacterial Expression ; E. coli/Gram-positive Bacterial Shuttle Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For E. coli, grow in Luria broth (LB) agar containing 50μg/mL Spectinomycin. For E. faecalis, select at 500μg/mL in culture and 1000μg/mL on plates.
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13_pUC_rev
  • 3′ sequencing primer M13_pUC_fwd; pBAD_rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDL278 was a gift from Gary Dunny (Addgene plasmid # 46882 ; http://n2t.net/addgene:46882 ; RRID:Addgene_46882)
  • For your References section:

    Molecular, genetic, and functional analysis of the basic replicon of pVA380-1, a plasmid of oral streptococcal origin. LeBlanc DJ, Lee LN, Abu-Al-Jaibat A. Plasmid. 1992 Sep;28(2):130-45. 10.1016/0147-619X(92)90044-B PubMed 1409970