|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46882||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene plasmid 46881
- Backbone size (bp) 6733
Modifications to backboneKanamycin resistance-encoding aphA3 gene and pVA380-1 ORF-1 of pDL276 replaced with spectinomycin resistance-encoding aad9 gene and pVA380-1 ORF-1 of pDL602 (see associated article for detailed construction information).
Vector typeBacterial Expression ; E. coli/Gram-positive Bacterial Shuttle Vector
Growth in Bacteria
Growth instructionsFor E. coli, grow in Luria broth (LB) agar containing 50μg/mL Spectinomycin. For E. faecalis, select at 500μg/mL in culture and 1000μg/mL on plates.
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13_pUC_rev
- 3′ sequencing primer M13_pUC_fwd; pBAD_rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDL278 was a gift from Gary Dunny (Addgene plasmid # 46882 ; http://n2t.net/addgene:46882 ; RRID:Addgene_46882)
For your References section:Molecular, genetic, and functional analysis of the basic replicon of pVA380-1, a plasmid of oral streptococcal origin. LeBlanc DJ, Lee LN, Abu-Al-Jaibat A. Plasmid. 1992 Sep;28(2):130-45. 10.1016/0147-619X(92)90044-B PubMed 1409970