STAT3(Y705F)-TAL-Luc
(Plasmid #46933)

• Purpose: Luciferase Reporter for mutant STAT3
• Depositing Lab: Afshin Dowlati
• Publication: Dabir et al Mol Cancer Res. 2009 Nov;7(11):1854-60. doi: 10.1158/1541-7786.MCR-09-0313. Epub 2009 Nov 10.

Backbone

• Vector backbone: pTAL-Luc
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 2351
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: STAT3(Y705F)
• Alt name: STAT3
• Alt name: signal transducer and activator of transcription 3 (acute-phase response factor)
• Species: H. sapiens (human)
• Insert Size (bp): 2351
• Mutation: Tyrosine 705 to Phenylalanine
• Entrez Gene: STAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
• Promoter: TA Luciferase
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: 5'-CGGGAGGTACTTGGAGCG-3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: George Stark Lab, Case Western Reserve University
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry