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pBabe TIR1-9myc
(Plasmid #47328)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47328 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe-puro
  • Total vector size (bp) 7300
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRANSPORT INHIBITOR RESPONSE 1
  • Alt name
    TIR1
  • Species
    O. sativa
  • Insert Size (bp)
    2091
  • GenBank ID
    NP_001052659.1
  • Tags / Fusion Proteins
    • NES (nuclear export signal - ALQKKLEELELDEGG) (N terminal on insert)
    • 9x Myc (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Nishimura et al., 2009, Nature Methods

Please note that this version contains a nuclear export signal. A related construct without the NES can be found at: http://www.addgene.org/64945/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe TIR1-9myc was a gift from Don Cleveland (Addgene plasmid # 47328 ; http://n2t.net/addgene:47328 ; RRID:Addgene_47328)
  • For your References section:

    Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells. Holland AJ, Fachinetti D, Han JS, Cleveland DW. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 10.1073/pnas.1216880109. Epub 2012 Nov 12. 10.1073/pnas.1216880109 PubMed 23150568