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pGEM-mcpGFP
(Plasmid #47442)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47442 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM T-easy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 3700
  • Modifications to backbone
    there are some residual attB1 and attB2 nucleotides in the linkers between pGEM and the insert.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mcpGFP
  • Alt name
    mutated circular permuted EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    723
  • Mutation
    M154K, V164A, S176G, D181Y, T204V, A207K, V190I

Cloning Information

Resource Information

Depositor Comments

same as
Tian L, Hires SA, Mao T, Huber D, Chiappe ME, Chalasani SH, et al. 2009. Imaging neural activity in worms, flies
and mice with improved GCaMP calcium indicators. Nat Methods 6:875–81. doi: 10.1038/nmeth.1398.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-mcpGFP was a gift from Wolf Frommer (Addgene plasmid # 47442 ; http://n2t.net/addgene:47442 ; RRID:Addgene_47442)
  • For your References section:

    Fluorescent sensors reporting the activity of ammonium transceptors in live cells. De Michele R, Ast C, Loque D, Ho CH, Andrade SL, Lanquar V, Grossmann G, Gehne S, Kumke MU, Frommer WB. Elife. 2013 Jul 2;2:e00800. Print 2013. PubMed 23840931