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Flag-Jun4A-Myc
(Plasmid #47444)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47444 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 47444-D50 50 μg of DNA in Tris buffer $495
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DNA 47444-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV-Tag2A
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Jun 4A mutant
  • Alt name
    Junc
  • Alt name
    c-Jun
  • Alt name
    AP-1
  • Species
    M. musculus (mouse)
  • Mutation
    four N-terminal residues phosphorylated by JNK, serines 63 and 73, and threonines 91 and 93, are mutated into alanines
  • Entrez Gene
    Jun (a.k.a. AP-1, Junc, c-jun)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-Jun4A-Myc was a gift from Axel Behrens (Addgene plasmid # 47444 ; http://n2t.net/addgene:47444 ; RRID:Addgene_47444)

  • For your References section:

    c-Jun N-terminal phosphorylation antagonises recruitment of the Mbd3/NuRD repressor complex. Aguilera C, Nakagawa K, Sancho R, Chakraborty A, Hendrich B, Behrens A. Nature. 2011 Jan 13;469(7329):231-5. doi: 10.1038/nature09607. Epub 2011 Jan 2. 10.1038/nature09607 PubMed 21196933
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