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EGFP-hPMCA2w/b
(Plasmid #47586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47586 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 47586-D50 50 μg of DNA in Tris buffer $495
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DNA 47586-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech; modified as described in Rogers et al JBC 2003
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PMCA2w/b
  • Alt name
    ATPase, Ca++ transporting, plasma membrane 2
  • Alt name
    ATP2B2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3754
  • Entrez Gene
    ATP2B2 (a.k.a. DFNA82, PMCA2, PMCA2a, PMCA2i)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP-HA-His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

w/b splice variant of human PMCA2. PMCA2w/b inserted as SacII-XbaI fragment into multiple cloning site but is also flanked by NotI sites. PMCA2 start: 1396; stop: 5125, w-splice: 2303

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-hPMCA2w/b was a gift from Emanuel Strehler (Addgene plasmid # 47586 ; http://n2t.net/addgene:47586 ; RRID:Addgene_47586)

  • For your References section:

    Alternative splicing of the first intracellular loop of plasma membrane Ca2+-ATPase isoform 2 alters its membrane targeting. Chicka MC, Strehler EE. J Biol Chem. 2003 May 16;278(20):18464-70. Epub 2003 Mar 6. 10.1074/jbc.M301482200 PubMed 12624087
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