RH1-bio
(Plasmid #47748)

• Purpose: Expresses enzymatically monobiotinylated partial RH1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
• Depositing Lab: Gavin Wright
• Depositing Organization: Wellcome Sanger Institute
  Located in United Kingdom of Great Britain and Northern Ireland
• Publication: Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 6633
• Total vector size (bp): 8562
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Codon-optimised RH1 (partial)
• Alt name: RBP1
• Alt name: NBP1
• Species: Synthetic; Plasmodium falciparum
• Insert Size (bp): 1928
• Mutation: Exogenous signal peptide of mouse origin, changed Serine72, Serine80, Serine89, Serine137, Serine288, Serine386 and Threonine419 to Alanine residues
• GenBank ID:
• Promoter: CMV
• Tags / Fusion Proteins:
  • rat Cd4 d3+4 (C terminal on backbone)
  • enzymatic biotinylation sequence (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV-fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained under an MTA from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 47748-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 47748-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.