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pMM2-hPMCA4a
(Plasmid #47761)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47761 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 47761-D50 50 μg of DNA in Tris buffer $495
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DNA 47761-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pMM2
  • Backbone manufacturer
    Adamo, H.P. et al. (1992) Biochem. J. 285, 791-797
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PMCA4a
  • Alt name
    ATP2B4
  • Alt name
    ATPase, Ca++ transporting, plasma membrane 4
  • Species
    H. sapiens (human)
  • Entrez Gene
    ATP2B4 (a.k.a. ATP2B2, MXRA1, PMCA4, PMCA4b, PMCA4x)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer pMT2-F
  • 3′ sequencing primer pMT2-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that PMCA4 residue 2 is Ala (instead of Thr) due to an optimized initiation sequence (ATG GCG). The alternatively spliced 178 nt exon sequence is at positions 4377-4554.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMM2-hPMCA4a was a gift from John Penniston & Emanuel Strehler (Addgene plasmid # 47761 ; http://n2t.net/addgene:47761 ; RRID:Addgene_47761)

  • For your References section:

    The Ca2+ affinity of the plasma membrane Ca2+ pump is controlled by alternative splicing. Enyedi A, Verma AK, Heim R, Adamo HP, Filoteo AG, Strehler EE, Penniston JT. J Biol Chem. 1994 Jan 7;269(1):41-3. PubMed 8276828
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