PurposeExpress full length HA tagged p35sr in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48184||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namep35srj (MRG1)
SpeciesH. sapiens (human)
Insert Size (bp)1027
Entrez GeneCITED2 (a.k.a. ASD8, MRG-1, MRG1, P35SRJ, VSD2)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HincII (unknown if destroyed)
- 5′ sequencing primer CMV-Fwd
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
HA tag added to 5'end of the cited2 cDNA using a blunt-ha-stop-xho oligo. The insert was ligated into the EcoRI/XhoI sites of pcDNA3
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SB51 was a gift from Shoumo Bhattacharya (Addgene plasmid # 48184 ; http://n2t.net/addgene:48184 ; RRID:Addgene_48184)
For your References section:Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1. Bhattacharya S, Michels CL, Leung MK, Arany ZP, Kung AL, Livingston DM. Genes Dev. 1999 Jan 1. 13(1):64-75. 10.1101/gad.13.1.64 PubMed 9887100