Purpose(Empty Backbone) sgRNA expression on PiggyBac vector with Neo-selectable marker
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48228||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
- Promoter U6
Selectable markersNeomycin (select with G418)
Growth in Bacteria
- Cloning method Restriction Enzyme
For more information including protocols and
updates, please go to http://www.crispr-on.org
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC103-PBNeo-U6-sgRNA-Expression was a gift from Rudolf Jaenisch (Addgene plasmid # 48228 ; http://n2t.net/addgene:48228 ; RRID:Addgene_48228)
For your References section:Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. 10.1038/cr.2013.122 PubMed 23979020