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PurposepH sensor
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Depositing Lab
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Depositing OrganizationUniversity of Geneva
Located in Switzerland -
Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48250 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 48250-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 48250-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepHyper-cyto
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Backbone manufacturerEvrogen
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSypHer
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SpeciesSynthetic
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Insert Size (bp)1400
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MutationC199S
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pHyper was cloned by replacing EGFP with the Hyper sequence in the pEGFP-C1 vector (Clontech). To make pSypHer, the C199S mutation was made with the QuikChange II site-directed mutagenesis kit using the following oligos:
5′-agatggtcactctttgcgcgat-3′ (forward)
5′-atcgcgcaaagagtgaccatct-3′ (reverse)
DNA (Catalog # 48250-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 48250-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SypHer was a gift from Nicolas Demaurex (Addgene plasmid # 48250 ; http://n2t.net/addgene:48250 ; RRID:Addgene_48250) -
For your References section:
Dynamic regulation of the mitochondrial proton gradient during cytosolic calcium elevations. Poburko D, Santo-Domingo J, Demaurex N. J Biol Chem. 2011 Apr 1;286(13):11672-84. doi: 10.1074/jbc.M110.159962. Epub 2011 Jan 11. 10.1074/jbc.M110.159962 PubMed 21224385