PurposeBacterial expression of Lacritin protein with 70 AA removed from the N-terminus
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48720||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerNew England BioLabs, INC
- Backbone size w/o insert (bp) 7477
- Total vector size (bp) 147
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsBacterial Strain was selected due to its ability to efficiently express mammalian protein (non-glycosolated) in bacterial cells.
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)147
MutationDeleted first 71 amino acids of mature lacritin without signal peptide
- Promoter T7
/ Fusion Protein
- Intein (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer ATGAAGTATCTTACTAAC
- 3′ sequencing primer TCATGCCCATGGTTTTAA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that amino acid numbering of this plasmid is relative to amino acid #20 in GenBank reference sequence NP_150593.1. E20 in the reference sequence is considered the first amino acid of lacritin in this plasmid.
Lacritin protein sequence in this plasmid is: SILLTEQALAKAGKGMHGGVPGGKQFIENGSEFAQKLLKKFSLLKPWA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTYB1-pLAC-N71 was a gift from Gordon Laurie (Addgene plasmid # 48720 ; http://n2t.net/addgene:48720 ; RRID:Addgene_48720)
For your References section:Lacritin rescues stressed epithelia via rapid forkhead box O3 (FOXO3)-associated autophagy that restores metabolism. Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler MF 4th, Lee JK, Laurie GW. J Biol Chem. 2013 Jun 21;288(25):18146-61. doi: 10.1074/jbc.M112.436584. Epub 2013 May 2. 10.1074/jbc.M112.436584 PubMed 23640897
Map uploaded by the depositor.