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Addgene

pGL2 Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid
48742 with the following enzymes: SalI-HF single digest, XhoI single digest, BamHI-HF single digest, and SalI-HF and XhoI double digest.

The linearized plasmid runs higher than expected (8kb vs 6kb). Addgene QC sequencing has verified the entire insert region.