PurposeProvides the alcA promoter as GreenGate module.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48817||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2686
Vector typeGolden Gate compatible cloning vector
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namealcA promoter
Insert Size (bp)246
- Cloning method Unknown
This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
alcA promoter = 2252-2497bp
BsaI site #2 = 2498-2508bp
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGGA008 was a gift from Jan Lohmann (Addgene plasmid # 48817 ; http://n2t.net/addgene:48817 ; RRID:Addgene_48817)
For your References section:GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J. PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PubMed 24376629