pBEG R3-miRNA(ccdB)-L4 #BGV228
(Plasmid #48953)

• Purpose: (Empty Backbone) To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).
• Depositing Lab: David Dankort
• Publication: Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279.

Backbone

• Vector backbone: pBEG R3-L4 (addgene plasmid 48954)
• Backbone manufacturer: Dankort Lab
• Backbone size (bp): 4058
• Modifications to backbone: A miRNA-30 cassette was synthesized by BioBasic and cloned into the NotI/EcoRV sites of pBEG R3-L4, then an EcoRI-XhoI flanked chloramphenicol ccdB cassette was cloned into the EcoRI-XhoI sites of the miRNA-30 cassette.
• Vector type: Gateway cloning vector
• Promoter: na

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified a few sequencing differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing lab, these differences are not a concern for the function of the plasmid.