sgRNA with U6 promoter
Purpose(Empty Backbone) to drive the sgRNA expression under a U6 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48962||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerlife technologies
Vector typeWorm Expression, CRISPR
- Promoter U6
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer GACGTAATACGACTCACTATAGGGC
- 3′ sequencing primer ACGACAGGTTTCCCGACTGG (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:sgRNA with U6 promoter was a gift from Mario de Bono (Addgene plasmid # 48962 ; http://n2t.net/addgene:48962 ; RRID:Addgene_48962)
For your References section:Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination. Chen C, Fenk LA, de Bono M. Nucleic Acids Res. 2013 Sep 5. 10.1093/nar/gkt805 PubMed 24013562