Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

FL MALT1 Catalytic Mutant in pET29b-Strep
(Plasmid #48969)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48969 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET29b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5371
  • Total vector size (bp) 7903
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MALT1 isoform A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2532
  • Mutation
    Catalytic Mutant C464A
  • Entrez Gene
    MALT1 (a.k.a. IMD12, MLT, MLT1, PCASP1)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (C terminal on backbone)
    • Strep (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FL MALT1 Catalytic Mutant in pET29b-Strep was a gift from Guy Salvesen (Addgene plasmid # 48969 ; http://n2t.net/addgene:48969 ; RRID:Addgene_48969)
  • For your References section:

    Mechanism and specificity of the human paracaspase MALT1. Hachmann J, Snipas SJ, van Raam BJ, Cancino EM, Houlihan EJ, Poreba M, Kasperkiewicz P, Drag M, Salvesen GS. Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035. 10.1042/BJ20120035 PubMed 22309193