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pBEG R2-iNeo-L3 #BGV207
(Plasmid #48986)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48986 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBEG R2+xL3 (Addgene plasmid 48952)
  • Backbone manufacturer
    Dankort Lab
  • Total vector size (bp) 3684
  • Vector type
    Gateway cloning vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    neomycin resistance
  • Promoter NA
  • Tag / Fusion Protein
    • IRES (weak) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEG R2-iNeo-L3 #BGV207 was a gift from David Dankort (Addgene plasmid # 48986 ; http://n2t.net/addgene:48986 ; RRID:Addgene_48986)
  • For your References section:

    A modular lentiviral and retroviral construction system to rapidly generate vectors for gene expression and gene knockdown in vitro and in vivo. Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, Dankort D. PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279. 10.1371/journal.pone.0076279 PubMed 24146852