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(Plasmid #48995)


Item Catalog # Description Quantity Price (USD)
Plasmid 48995 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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  • Vector backbone
  • Backbone manufacturer
    Life Technologies (Invitrogen)
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 2900
  • Vector type
    Gateway attB1 & attB2
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Sh ble
  • Alt name
    Phelomycin resistance gene
  • Insert Size (bp)

Cloning Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results found a few differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing laboratory, these differences are not a concern for the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-Phelo was a gift from Rick Tarleton (Addgene plasmid # 48995 ; ; RRID:Addgene_48995)
  • For your References section:

    Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi. Xu D, Brandan CP, Basombrio MA, Tarleton RL. BMC Microbiol. 2009 May 11;9:90. doi: 10.1186/1471-2180-9-90. 10.1186/1471-2180-9-90 PubMed 19432966