645 pCS2-etv2
(Plasmid
#49005)
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PurposeMammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2
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Depositing Lab
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Depositing OrganizationUniversity of Massachusetts, Worcester
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49005 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 49005-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 49005-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS-dest
- Total vector size (bp) 5259
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Vector typeMammalian Expression ; invitro transcription, riboprobe template
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameets variant gene 2
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Alt nameetv2
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1133
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Entrez Geneetsrp (a.k.a. ets1b, etv2, grc, wu:fc49b07, zgc:153819)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer Sp6
- 3′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 49005-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 49005-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
645 pCS2-etv2 was a gift from Nathan Lawson (Addgene plasmid # 49005 ; http://n2t.net/addgene:49005 ; RRID:Addgene_49005) -
For your References section:
Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N. Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 10.1016/j.ydbio.2013.08.028. 10.1016/j.ydbio.2013.08.028 PubMed 24036310
