PurposeAAV plasmid with CLDN5 (Ple261) promoter driving expression of iCre.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49113||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
- Promoter CLDN5
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
Please see the following publication for more information:
rAAV-compatible MiniPromoters for restricted expression in the brain and eye.
de Leeuw et al. Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4. PMID 27164903
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEMS1982 was a gift from Elizabeth Simpson (Addgene plasmid # 49113 ; http://n2t.net/addgene:49113 ; RRID:Addgene_49113)
For your References section:Ferroportin-mediated iron export from vascular endothelial cells in retina and brain. Baumann BH, Shu W, Song Y, Simpson EM, Lakhal-Littleton S, Dunaief JL. Exp Eye Res. 2019 Jul 16:107728. doi: 10.1016/j.exer.2019.107728. 10.1016/j.exer.2019.107728 PubMed 31323276