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pEMS2022
(Plasmid #49129)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49129 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ssAAV-PRS2/3:Con-iCre
  • Alt name
    iCre
  • Species
    Synthetic
  • Promoter DBH

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Depositor Comments

For additional information, please see the following publications:

Hwang DY et al., 2005. Genetically engineered dopamine beta-hydroxylase gene promoters with better phox2-binding sites drive significantly enhanced transgene expression in a noradrenergic cell-specific manner. Mol Ther 11, 132-141, PMID 15585414 (https://www.ncbi.nlm.nih.gov/pubmed/15585414).

de Leeuw CN et al., rAAV-compatible MiniPromoters for restricted expression in the brain and eye. Mol Brain. 2016 May 10;9(1):52, PMID 27164903 (https://www.ncbi.nlm.nih.gov/pubmed/27164903).

Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS2022 was a gift from Elizabeth Simpson (Addgene plasmid # 49129 ; http://n2t.net/addgene:49129 ; RRID:Addgene_49129)
  • For your References section:

    rAAV-compatible MiniPromoters for restricted expression in the brain and eye. de Leeuw CN, Korecki AJ, Berry GE, Hickmott JW, Lam SL, Lengyell TC, Bonaguro RJ, Borretta LJ, Chopra V, Chou AY, D'Souza CA, Kaspieva O, Laprise S, McInerny SC, Portales-Casamar E, Swanson-Newman MI, Wong K, Yang GS, Zhou M, Jones SJ, Holt RA, Asokan A, Goldowitz D, Wasserman WW, Simpson EM. Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4. 10.1186/s13041-016-0232-4 [pii] PubMed 27164903