PurposeAAV plasmid with FEV (Ple67) promoter driving expression of EmGFP.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49139||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
- Promoter FEV
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEMS2114 was a gift from Elizabeth Simpson (Addgene plasmid # 49139 ; http://n2t.net/addgene:49139 ; RRID:Addgene_49139)
For your References section:Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain; Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM. Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 10.1534/genetics.119.301984. 10.1534/genetics.119.301984 PubMed 30765420