Purpose(Empty Backbone) Plasmid for cloning spacer into sgRNA for NmCas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49157||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 1788
Vector typeMammalian Expression, CRISPR
- Promoter U6
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CAG TCC AGT TAC GCT GGA GTC (Common Sequencing Primers)
Use enzyme BbsI for cloning in the sgRNA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSmart-Nm-sgRNA-BbsI was a gift from James Thomson (Addgene plasmid # 49157 ; http://n2t.net/addgene:49157 ; RRID:Addgene_49157)