pLJM1-STAG2
(Plasmid #49162)

• Purpose: Expression of wild-type STAG2 from a lentiviral vector
• Depositing Lab: Todd Waldman
• Publication: Solomon et al Nat Genet. 2013 Oct 13. doi: 10.1038/ng.2800.

Backbone

• Vector backbone: pLJM1
• Backbone manufacturer: David Sabatini
• Backbone size w/o insert (bp): 7200
• Total vector size (bp): 11200
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Grow in XL10 Gold or Stbl2 cells
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STAG2
• Species: H. sapiens (human)
• Insert Size (bp): 3696
• GenBank ID: NM_001042751.1
• Entrez Gene: STAG2 (a.k.a. SA-2, SA2, SCC3B, bA517O1.1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry