ME2/pBABE-puro
(Plasmid #49164)

• Purpose: For expression of Malic Enzyme 2 in mammalian cells from retroviral vector
• Depositing Lab: Xiaolu Yang
• Publication: Jiang et al Nature. 2013 Jan 31;493(7434):689-93. doi: 10.1038/nature11776. Epub 2013 Jan 13.

Backbone

• Vector backbone: pBabe-puro
• Backbone manufacturer: Addgene plasmid # 1764
• Backbone size w/o insert (bp): 5169
• Total vector size (bp): 6924
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Malic Enzyme 2
• Alt name: ME2
• Species: H. sapiens (human)
• Insert Size (bp): 1755
• Entrez Gene: ME2 (a.k.a. ODS1)
• Promoter: SV40

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: pBABE-5
• 3′ sequencing primer: pBABE-3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry