pPCR-Script-mC/EBPb
(Plasmid
#49195)
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Purposeinitial cloning vector for C/EBPbeta
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49195 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepPCR-Script Amp SK(+)
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2961
- Total vector size (bp) 3800
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Vector typeBacterial Expression ; cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameC/EBPbeta
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Alt nameCCAAT/enhancer binding protein (C/EBP), beta
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Alt nameLAP
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SpeciesM. musculus (mouse)
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Insert Size (bp)853
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Entrez GeneCebpb (a.k.a. C/EBPbeta, CRP2, IL-6DBP, LAP, LIP, NF-IL6, NF-M, Nfil6)
- Promoter lac
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPCR-Script-mC/EBPb was a gift from Jed Friedman (Addgene plasmid # 49195)