Flag‐hTim1
(Plasmid #49207)

• Purpose: expresses Flag-tagged human Tim1
• Depositing Lab: Lawrence Kane
• Publication: de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):17113-8. Epub 2005 Nov 11.

Backbone

• Vector backbone: pCDEF3-CD8SS-Flag
• Backbone manufacturer: Kane Lab
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Tim1
• Alt name: hepatitis A virus cellular receptor 1
• Alt name: Havcr1
• Species: H. sapiens (human)
• Mutation: Tim1 lacks its own signal sequence
• Entrez Gene: HAVCR1 (a.k.a. CD365, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1)
• Promoter: EF1a
• Tags / Fusion Proteins:
  • signal sequence (N terminal on backbone)
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cDNA PCR amplified from Integrated Molecular Analysis of Genomes and their Expression (I.M.A.G.E.) consortium clone #3826378