Purposeretroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49267||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backboneMSCV 2.2 IRES GFP
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesB. taurus (bovine)
Insert Size (bp)1500
MutationN2D, D149E and A478G compared to AAW71957.1
Entrez GeneAKT1 (a.k.a. BOS_20453)
/ Fusion Proteins
- myr (N terminal on insert)
- IRES-EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pLXSN-5
- 3′ sequencing primer pCDH-rev (GCATTCCTTTGGCGAGAG) (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that Addgene's sequencing results identified N2D, D149E and A478G mutations in the Akt sequence in this plasmid when compared to GenBank reference sequence AAW71957.1. These mutations were not intentional and the plasmid functions as described in the associated publication (i.e. it is constitutively active).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV-myrAkt was a gift from Lawrence Kane & Arthur Weiss (Addgene plasmid # 49267 ; http://n2t.net/addgene:49267 ; RRID:Addgene_49267)
For your References section:Akt provides the CD28 costimulatory signal for up-regulation of IL-2 and IFN-gamma but not TH2 cytokines. Kane LP, Andres PG, Howland KC, Abbas AK, Weiss A. Nat Immunol. 2001 Jan;2(1):37-44. 10.1038/83144 PubMed 11135576
Map uploaded by the depositor.