|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49392||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3900
Vector typeMammalian Expression, Cre/Lox ; donor vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Copy numberHigh Copy
Alt namehuman Sry
SpeciesH. sapiens (human)
Insert Size (bp)953
MutationTGA (stop) to CCA (Pro) at nt 1027 with respect to GenBank ID BC013923
Entrez GeneSOX2 (a.k.a. ANOP3, MCOPS3)
- Promoter Tet-responsive
/ Fusion Protein
- 6xHN (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer LNCX_primer (Common Sequencing Primers)
Terms and Licenses
hSox2 was transferred from the donor vector (pDNR-hSox2, Addgene plasmid #49389) to the acceptor vector using Cre-mediated recombination at the LoxP sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLP-TRE2-hSox2 was a gift from Randall McKinnon (Addgene plasmid # 49392 ; http://n2t.net/addgene:49392 ; RRID:Addgene_49392)
Map uploaded by the depositor.