Purposemammalian bicistronic expression of human Sox2 and EGFP
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49391||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression, Cre/Lox ; acceptor vector
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
Copy numberHigh Copy
Alt namehuman Sry
SpeciesH. sapiens (human)
Insert Size (bp)953
MutationTGA (stop) to CCA (Pro) at nt 1027 with respect to GenBank ID BC013923
Entrez GeneSOX2 (a.k.a. ANOP3, MCOPS3)
- Promoter CMV
/ Fusion Proteins
- 6xHN (C terminal on backbone)
- IRES (C terminal on backbone)
- EGFP (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
hSox2 was transferred from the donor vector (pDNR-hSox2, Addgene plasmid #49389) to the acceptor vector using Cre-mediated recombination at the LoxP sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLP-hSox2-ires-gfp was a gift from Randall McKinnon (Addgene plasmid # 49391 ; http://n2t.net/addgene:49391 ; RRID:Addgene_49391)
Map uploaded by the depositor.