pUC21
(Plasmid
#49787)
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Purpose(Empty Backbone) E. coli cloning vector (AmpR, high copy, blue/white selection, M13 IR)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneunknown
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Backbone manufacturerMessing Lab
- Backbone size (bp) 3200
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is analogous to pUC118/119 (Vieira and Messing 1987); it carries the bla gene coding for Ap resistance, the M13 IR allowing for the production of ss plasmid DNA, and the MCS inserted within the N-terminal end of the lacZalpha gene allowing white/blue screening for inserts.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC21 was a gift from Joachim Messing (Addgene plasmid # 49787 ; http://n2t.net/addgene:49787 ; RRID:Addgene_49787) -
For your References section:
New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Vieira J, Messing J. Gene. 1991 Apr;100:189-94. 10.1016/0378-1119(91)90365-I PubMed 1905257
Map uploaded by the depositor.
