|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50009||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerMessing Lab
Modifications to backboneIG region of M13 from the HgiAI site (5465) to the DraI site (5941) inserted at the unique NdeI site (2499) of pUC. The orientation of the M13 IG region is such that the lac region that is packaged as ssDNA is the same as in the M13mp vectors.
Vector typecloning vector
Growth in Bacteria
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC118 was a gift from Joachim Messing (Addgene plasmid # 50009 ; http://n2t.net/addgene:50009 ; RRID:Addgene_50009)
For your References section:Production of single-stranded plasmid DNA. Vieira J, Messing J. Methods Enzymol. 1987;153:3-11. PubMed 3323803