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pUC118
(Plasmid #50009)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50009 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC18
  • Backbone manufacturer
    Messing Lab
  • Modifications to backbone
    IG region of M13 from the HgiAI site (5465) to the DraI site (5941) inserted at the unique NdeI site (2499) of pUC. The orientation of the M13 IG region is such that the lac region that is packaged as ssDNA is the same as in the M13mp vectors.
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC118 was a gift from Joachim Messing (Addgene plasmid # 50009 ; http://n2t.net/addgene:50009 ; RRID:Addgene_50009)
  • For your References section:

    Production of single-stranded plasmid DNA. Vieira J, Messing J. Methods Enzymol. 1987;153:3-11. PubMed 3323803