Purposeexpresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50051||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6200
Modifications to backboneThe vector is modified for LIC cloning, but the insert can also be extracted using 5' Nhe I/sal I/BsiW1, and 3' Spe I, Asc I/BssH II.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Alt nameMIC2 associated protein
Insert Size (bp)900
Mutationstarts at aa47; N127R, S158A, S177N, N357S, N463S, N470D
- Promoter CMV
/ Fusion Protein
- HIS (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byDr. Vern B. Carruthers University of Michigan Medical School
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The first 46aa consist of a pro-peptide that gets cleaved during maturation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:m2ap was a gift from Timothy Springer (Addgene plasmid # 50051 ; http://n2t.net/addgene:50051 ; RRID:Addgene_50051)