m2ap
(Plasmid #50051)

• Purpose: expresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells
• Depositing Lab: Timothy Springer
• Publication: Springer lab MIC2 plasmid (unpublished)

Backbone

• Vector backbone: pIRES2-EGFP
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6200
• Modifications to backbone: The vector is modified for LIC cloning, but the insert can also be extracted using 5' Nhe I/sal I/BsiW1, and 3' Spe I, Asc I/BssH II.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: M2AP
• Alt name: MIC2 associated protein
• Species: toxoplasma gondii
• Insert Size (bp): 900
• Mutation: starts at aa47; N127R, S158A, S177N, N357S, N463S, N470D
• GenBank ID: AAK51546.1
• Promoter: CMV
• Tag / Fusion Protein:
  • HIS (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: IRES-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Vern B. Carruthers University of Michigan Medical School

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The first 46aa consist of a pro-peptide that gets cleaved during maturation.