|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50333||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeplant expression
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameBiotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))
MutationBsaI/BbsI sites removed by point-mutation
- Cloning method Restriction Enzyme
- 5′ cloning site BpiI (destroyed during cloning)
- 3′ cloning site BpiI (destroyed during cloning)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
insert can be released with BsaI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pICSL70002 was a gift from Nicola Patron (Addgene plasmid # 50333 ; http://n2t.net/addgene:50333 ; RRID:Addgene_50333)
For your References section:A golden gate modular cloning toolbox for plants. Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, Patron NJ, Marillonnet S. ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. Epub 2014 Feb 20. 10.1021/sb4001504 PubMed 24933124