PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50594||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typePlant Expression, CRISPR ; PCR template
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
Copy numberHigh Copy
Gene/Insert nameT2, gRNA scaffold, TaU3t plus, U6-26p, T3
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCBC-MT2T3 was a gift from Qi-Jun Chen (Addgene plasmid # 50594 ; http://n2t.net/addgene:50594 ; RRID:Addgene_50594)
For your References section:A CRISPR/Cas9 toolkit for multiplex genome editing in plants. Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ. BMC Plant Biol. 2014 Nov 29;14(1):327. 10.1186/s12870-014-0327-y PubMed 25432517