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Addgene

pCYP119
(Plasmid #50771)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50771 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC18
  • Backbone size w/o insert (bp) 2600
  • Total vector size (bp) 3800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CYP119
  • Species
    Sulfolobus solfatarius
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco R1 (not destroyed)
  • 3′ cloning site Bam H1 (not destroyed)
  • 5′ sequencing primer M13 reverce
  • 3′ sequencing primer M13
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCYP119 was a gift from Stephen Sligar (Addgene plasmid # 50771 ; http://n2t.net/addgene:50771 ; RRID:Addgene_50771)
  • For your References section:

    Characterization of a cytochrome P450 from the acidothermophilic archaea Sulfolobus solfataricus. McLean MA, Maves SA, Weiss KE, Krepich S, Sligar SG. Biochem Biophys Res Commun. 1998 Nov 9;252(1):166-72. 10.1006/bbrc.1998.9584 PubMed 9813164