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(Plasmid #27394)


Item Catalog # Description Quantity Price (USD)
Plasmid 27394 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
    E. coli
  • Entrez Gene
    groL (a.k.a. b4143, ECK4137, groEL, mopA)
  • Entrez Gene
    groS (a.k.a. b4142, ECK4136, groES, mopB)

Cloning Information

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Dr. Bernd Bukau, ZMBH, University of Heidelberg
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.

de Marco A.

Nat Protoc. 2007;2(10):2632-9.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBB541 was a gift from Bernd Bukau (Addgene plasmid # 27394 ; ; RRID:Addgene_27394)
  • For your References section:

    Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli. de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B. BMC Biotechnol. 2007 . 7():32. 10.1186/1472-6750-7-32 PubMed 17565681